GROWTH CONTROL OF DIFFERENTIATED FETAL RAT HEPATOCYTES IN PRIMARY MONOLAYER CULTURE
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چکیده
منابع مشابه
Growth Control of Differentiated Fetal Rat Hepatocytes in Primary Monolayer Culture
The initiation of DNA synthesis has been studied in quiescent primary cultures of fetal rat hepatocytes using defined hormones and chemically defined medium. Preparations of crystalline insulin (0.01-10 microg/ml) or 20,000-fold purified somatomedin (0.01-1 microg/ml) are stimulatory. Growth hormone (0.025 microg/ml) and hydroxycortisone (0.025 microg/ml), 3':5'-GMP! (10(-5) M) fail by themselv...
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INTRODUCTION pregnant and growing (neonatal) rats (18, 19) as well as in the serum of adult rats induced by partial Serum proteins associated with malignancies ("onhepatectomy to regenerate their liver (20, 21) . coproteins") have attracted considerable attention, These observations imply that biosynthesis and especially because of their possible use in the early release of a 1 F are related to...
متن کاملMaintenance of differentiated rat hepatocytes in primary culture.
Normal adult rat hepatocytes remained viable and functional for at least 43 days when plated on collagen-coated dishes and fed chemically defined medium supplemented with dimethyl sulfoxide (Me2SO). Hepatocytes isolated by collagenase perfusion and cultured in the presence or absence of Me2SO were (i) examined by light and electron microscopy for morphological changes; (ii) analyzed for the pro...
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Primary monolayer cultures were obtained in 60-mm petri dishes by incubating 3 X 10(6) isolated hepatocytes at 37 degrees C in Dulbecco's medium supplemented with 17% fetal calf serum. The ultrastructure of monolayer cells was examined after various incubation periods. Within 4 h of plating, the isolated spherical cells adhere to the plastic surface, establish their first contacts by numerous i...
متن کاملDevelopment of fetal rat intestine in organ and monolayer culture
Maturation and differentiation of intestinal epithelial cells was demonstrated in segments of fetal rat small intestine, maintained for more than a month in suspension organ culture, by ultrastructural, biochemical, and immunological criteria. Over a 5-7 d period, fragments of fetal intestine evolved into globular structures covered with a single columnar epithelium ultrastructurally similar to...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1974
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.62.3.792